Amylase lab report Essays

Amylase lab report Essays Amylase lab report Paper Amylase lab report Paper Enzymes are very specific; for example, amylase is the only enzyme that will break down starch. It is similar to the theory of the lock and the key. The enzyme is the lock and the key is the substrate; only the correct key could fit into the keyhole of the lock. Porcine pancreatic amylase is The purpose of this lab experiment is to investigate factors that can affect the porcine pancreatic amylase enzyme activity in different environments such as the temperature, pH and also how being stored in extreme temperatures can affect the activity of the amylase. The activity of the amylase is going to be determined by the presence or absence of starch in the samples over time. There are some hypotheses on the Effects of temperature and pH; as I add the amylase to the starch in different temperatures the reactions rate increases in high temperatures; I believe that the amylase will work better. As the environment grows warmer, the amylase is going to become more energetic and more effective. Amylase is affected by environmental PH. Predict that the amylase activity will work best at a pH 7. As the pH changes from this point I predicted that the amylase activity is going to decrease and eventually stop. If I boiled and rose some amylase solution, and try to digest starch with at it at room temperature, I predict the previously-boiled and frozen amylase will not work quite as well as amylase that has not been previously boiled and frozen. Experiment #1: Investigating the Effect of Environmental Temperature on the Activity of Porcine Pancreatic Amylase Materials and Methods Amylase experiment #1 was done to see how the temperature affected the efficacy of the enzyme. First we collected all of the materials that were necessary to perform this experiment. We needed five clean test tubes, a test tube rack, a beaker of 1 % starch solution (pH 7. ), a test tube of porcine amylase solution which was previously prepared by mixing porcine pancreatic amylase powder with 0. 9% Nasal concentration to create an amylase concentration of 0. Magma/ ml, one I-ml pipette, one 5-ml pipette with pipette pump, five transfer pipettes, two spot plates, a bottle of iodine solution, a timer, one beaker half way filled with ice and a small amount of water, two thermometers, and three water baths each with a different temperature. In this experiment, we first labeled the test tubes. We then added 5-ml of 1% starch solution with a pH of 7. 0 to each of them. After each tube was filled, the first one was placed in the freezer at O degrees Celsius, the second one was placed in room temperature with a temperature of 20 degrees Celsius, the third one was placed in warm water at 40 degrees Celsius, the fourth one was placed in hot water at 60 degrees Celsius, and fifth one was placed in extremely hot water at 80 degrees Celsius. Each test tube was then left in the selected place for ten minutes so that the starch solution could acclimate to its surrounding temperature. After ten minutes, we then used a 1 -ml pipette to add 0. Ml of the amylase mixture to each of the tubes and then the timer was set. After three minutes, we put 3 drops of the starch/amylase mixture from each of the five tubes into the spot plate, and then added two drops of iodine to each spot plate and noted the result. Iodine reacts with starch to change from yellow (not starch) to deep blue black in the presence of starch. After every three minutes had passed, these same steps were repeated, until 30 minutes had passed and noted all the result. Experiment #2: Investigating the Effect of Environmental pH on the Activity of Porcine Pancreatic Amylase Materials and Methods Amylase experiment # 2 was done to see how the pH affected the efficacy f the enzyme. First we collected all of the materials that were necessary to make this experiment. We needed five clean test tubes, the following standard solutions, 1% Starch Solution pH 3, 1% Starch Solution pH 5,1% Starch Solution pH 7,1% Starch Solution pH 9, 1% Starch Solution pH 11 0. 0375 MGM/ml Porcine Pancreatic Amylase Solution (amylase powder in 0. % Nasal ), Iodine Solution; each solution were pipettes into each of the 5 test tubes with 5 ml of 1% starch. Each tube contained a 1% starch solution with a different PH. All tubes were at room temperature. Room temperature was ICC. 0. 2 ml of porcine pancreatic amylase solution was then pipettes into each tube. A timer was started and every minutes the starch / amylase mixture were pipettes from each tube and pipettes into the spo t plate for every sample tube, then the iodine solution were added to a spot plate cell for each sample. Iodine reacts with starch to change from yellow to deep blue /black in the presence of starch. A lightening of the blue/ black to a brown color will occur as less starch is present. Results were reported as (+) for presence of starch in the sample or (-) for the absence of starch. After every three minute increment had passed, these same steps ere repeated, until 30 minutes had passed and all the results were noted. Experiment #3: Investigating the Effect of Freezing and of Boiling on the Activity of Porcine Pancreatic Amylase Materials and Methods Amylase experiment #3 was made to see if boiling or freezing the amylase would have an effect on the enzyme. For this experiment, we collected three clean test tubes, porcine pancreatic amylase solution, a test tube containing frozen amylase solution, two 600-ml beakers each containing 200-ml of room temperature water, a hot plate, two transfer pipettes, two spot plates, a bottle of iodine solution, First we took the frozen amylase solution out of the freezer and warmed it to room temp. Then we put about 0. Ml of amylase solution in a test tube and boiled it in at beaker for two minutes. Then we let the beakers sit in room temperature. We labeled each test tube, one boiled and the other frozen. We placed them in water and allowed them to sit for at least ten minutes until it cooled down to room temperature. Then we put 5-ml of starch solution pH 7. 0 in two test tubes and labeled them boiled amylase, or frozen amylase. We used a ml pipette to add 0. Ml of the boiled and frozen amylase solutions to their exceptive test tubes. The solutions were then thoroughly mixed together with a transfer pipette and the timer was set. After three minutes, we did the first test. We took out approximately three drops of starch/amylase solution from all two of the test tubes and put them on a spot plate into two separate wells. Then about two drops of iodine solution were added to each one. This same routine was repeated every three minute intervals until thirty minutes all together had passed. All of these tests were noted prior to each one. Discussion In the first experiment my hypothesis was that the amylase is going to work deter. As the environment gets warmer, and is going to become more active and get more effective. The amylase was put in different temperatures and the results were that amylase best works at a temperature near body temperature. While when the temperature starts decreasing to successes or increase to Celsius the amylase will work, but not at its 100 %, but when temperature reach Celsius or Celsius amylase dont work at all. The results of the experiments showed a significant effect of temperature on the amylase activity. The best temperature for porcine pancreatic amylase activity was ICC; it takes 6 min to gets the starch, as the temperature changed, the amylase activity decreased. When ICC was reached the amylase takes 12 min digesting the starch, also when ICC was reached the amylase took mini digesting the starch. Amylase is affected by environmental PH. I predict that the amylase activity will work best at pH 7. As the pH changes from this point I believe that the amylase activity is going to decrease and eventually stop. In experiment #2 the amylase was added into different pH to determine the optimum pH range. The result was that porcine pancreatic enzymes have best efficiency in the neutral pH 7 range, and does to work at its 100% potential at either between pH 9 and phi. The amylase did not work at all in extremely high or low pH such as phi and pH 11 . The results indicate that in pH 7 the enzyme works at its best. This can indicate that the amylase activity is optimal at pH ranges of the saliva and the small intestine. The two extreme pH values of pH 3 and pH 11 showed no amylase activity. This could be due to the anticipated competition at the enzyme active sites with increased hydrogen ion or hydroxide ion concentrations. Also the attraction of H or OH ions to the protein functional groups can alter the shape of the enzyme and thereby emit its activity. If I boiled or froze some amylase solution, and tried to digest starch with at it at room temperature, I predict the previously-boiled or frozen amylase will not work quite as well as amylase that has not been previously boiled or frozen. In experiment #3 the amylase activity was zero in the boiled sample. This confirms the prediction that freezing the amylase is not going to stop it from functioning, but is not going to work quite as well. In contrast the boiled sample results weakened my hypothesis because the amylase never showed any activity during the thirty min of the experiment. This can be imparted to the results in experiment #1 because amylase had no activity before being boiled, and neither when being in temperatures too high such as ICC.

A Case Study About Healthcare Leadership

A Case Study About Healthcare Leadership Effective leadership is essential in health care organisations as in other organisations. It is necessary for driving innovation, effective patient care, patient safety, improving working within clinical teams, sorting out issues within emergency context and other aspects necessary for effective and efficient running of healthcare organisations. Transformational leadership has often been prescribed as the ‘gold standard’ of healthcare leadership(Gopee and Galloway 2009).This essay sets out to: briefly discuss the concept of leadership; highlight why leadership is important in healthcare; make a distinction between the closely-related concepts of leadership and management; briefly highlight how power relates to leadership; describe some leadership approaches applicable within the context of healthcare organisations; describe leadership styles visible in healthcare; present a case study set in a teaching hospital practice setting in Africa ; critically assess the leadersh ip approaches operating within the setting and its effect on organisational performance ;and make recommendations on improving leadership practice within the specified setting. What is leadership? Leadership can be defined as â€Å"the ability of an individual to influence a group of people to achieve a goal(Bryman 1992).It is also noted that ‘leadership can have four possible meanings, namely: the activity of leading; the body of people who lead a group; the status of the leader; and the ability to lead'(Gopee and Galloway 2009). Kouzes and Posner (2007) suggest some characteristics of an effective leader namely to: be more effective in meeting job-related demands; be more successful in representing their units in upper management; create higher performing teams; foster renewed loyalty and commitment; increase motivational levels and willingness to work hard; and possess high degree of personal integrity. Why leadership in healthcare? Effective leadership and management has been found to contribute to efficiency of health care services, performance (McColl-Kennedy and Anderson 2002) and satisfaction of staff employed within them.(Bradley and Alimo-Metcalfe 2008) researched the causal relationship between leadership behaviours and the performance and productivity of staff and found that ‘engaging leadership’ improved employee engagement and performance. (Morrison, Jones et al. 1997) studied the relationship between leadership style, empowerment, and job satisfaction on nursing staff at a regional medical centre. They used Bass’s Multifactor Leadership Questionnaire to measure leadership style, items from Spreitzer’s Psychological Empowerment instrument to measure empowerment, and the Warr, Cook, and Wall’s job satisfaction questionnaire to measure job satisfaction. The authors found that both transformational and transactional leadership were positively associated with job satisfaction. Some other researchers reported t hat good leadership skills impacted on patient safety and quality of care (Corrigan, Lickey et al. 2000; Firth-Cozens and Mowbray 2001; Mohr, Abelson et al. 2002).Furthermore, leadership skills are essential in the world of public health policy and leadership is one of the core competencies required of public health trainees(Faculty of Public Health 2010). Leadership versus management Relevant to this discourse is making a distinction between leadership and management. They are two similar but distinct concepts. Management is seen as seeking order and maintaining stability while leadership is seen as seeking adaptive and constructive change. Leadership in the healthcare context aims to influence practitioners towards the achievement of the common goal of quality patient care. On the other hand, management as a process coordinates and directs the activities of an organisation to ensure it achieves its set objectives. Management ensures healthcare resources (human such as doctors, nur ses and clerical staff and non-human resources like medical devices and consumables) are utilised in an efficient way whilst delivering effective healthcare service(Gopee and Galloway 2009). However, leadership is known to be complementary to management (Kotter 1999; Zaleznik 2004).

Latest innovations Research Paper Example | Topics and Well Written Essays - 1250 words

Latest innovations - Research Paper Example Innovation is critical since it not only raises market attention but also helps in positioning and differentiation (OFallon, & Rutherford 2011). Innovation involves a new way of doing things using unique combination of production or delivery factors. Innovation is applicable in different ways like generation of a new product or improving an old product; the hotels can also develop new sales and supply markets or even restructure the whole company. With an aim of insuring sustainable development, the hotel industry is now adopting the new trend of eco-tourism. With years to come, all hotels will have legal obligation to ensure that all their practices aim at environmental conservation (Swami, & Sharma 2011). Hotels adopting eco-friendly innovations are beginning to have competitive advantage over other companies that do not protect the environment. Green hotels are growing daily hence becoming the core of the hospitality industry. Customers to this industry are also becoming eco sensitive hence are considering green hotel to meet their accommodation needs. The Eco label is becoming a standard for choice by the tourists and other hotel guests. Eco label hotels add environment friendly technological practices to their good menus besides providing a wide range of resources (Swami, & Sharma 2011). In the hotel environment, various activities such as constructions, landscaping, use of water and energy, cooking and disposal of waste s affect the environment if not contained. The size of hotel families in relation to the rate of utilization also influences environmental conditions (Swami, & Sharma 2011). There are varieties of forces that are pushing US hotels to be more environmental conscious. Some studies indicate that 90% of the current hotels guest aim at getting accommodations form Eco labelled hotels (Swami, & Sharma 2011). This causes pressure on hotels to adopt environment friendly techniques to maintain

Intro to law Essay Example | Topics and Well Written Essays - 250 words

Intro to law - Essay Example platform when two men ran in to catch the train the second man carried luggage and appeared to be falling when the train employees or guards who were standing by moved in to help him by pushing him into the train. In the course of the entry, the luggage of the second man fell down (it had fireworks), so it exploded hitting the tracks (Prosser, 1953). The impact on the tracks initiated the scales on the other end of the train to fall thereby injuring the plaintiff Mrs. Helen Palsgraf. The plaintiff sued the railroad claiming she was injured due to the negligence of the employees of the railroad company. Judgment: the bench by way of majority dismissed the application of the plaintiff and instead arguing that the actions of the accused employee were too indirectly linked to the injury caused to the plaintiff. In principle, there was no way he could have known what the passenger and owner of the luggage had carried. Thus, the case was dismissed with

Critical Perspectives on Management Essay Example | Topics and Well Written Essays - 3250 words

Critical Perspectives on Management - Essay Example This research investigated the Derrida's views on the worth of deconstruction for management students and vital aspects of deconstruction between managerial decision-making of managers and a set of specified contingent situational training factors: decision type, perceived skill requirements, and objective skill inputs (Bowie N. E.,2002). The term deconstruction was coined by French philosopher Jacques Derrida in the 1960s and is used in contemporary humanities and social sciences to denote a philosophy of meaning that deals with the ways that meaning is constructed by writers, texts, and readers and understood by readers (Campbell J., 2002). One way of understanding the term is that it involves discovering, recognizing, and understanding the underlying and unspoken and implicit assumptions, ideas, and frameworks that form the basis for thought and belief. It has various shades of meaning in different areas of study and discussion, and is, by its very nature, difficult to define with out depending on "un-deconstructed" concepts (Ciulla J. B., 2005, 5-28). The results show a significant training for management student and relationship between the choice of decision styles and the postulated contingency variables. Only 1% of 615 senior managers consistently use a single decision style, more than two-thirds use four or five different styles. Very large variations occur as a function of different decision tasks, perceived skill requirements and objective skill availability (Cropanzano R.).According to the expert analysis, Derrida has been called a philosopher, anti-philosopher, fictional theorist, fictional subverter and thinker joker. But his innermost doctrines are clear. For management students, his conceptions are a source of excel once we use language (speech or writing) to submit to actuality, that realism is linguistically formulated and therefore undetermined. Import is not impressive preexisting in the mind that we move violently to articulate. Like the maj or logical schools of management students for talking philosophy from Hume onwards, and different from Saussure, Derrida does not consider words as the appearance of ideas (Bowie N. E.,2002).

Discuss the expression theory of art, using the chapter from Essay

Discuss the expression theory of art, using the chapter from freelandbut is it art - Essay Example Sometimes these theories focus solely upon the creation process of the artwork while other theories focus more upon the receiving process of the audience. Still other theories range around the concepts between these extremes, from the way in which the thoughts, feelings, ideas or other communicated goals are transferred from the artist’s mind to the canvas, musical score or movement, captured within that medium and then transferred to an audience. As is evident from the range of these theories, the role of the individual artist has been explored, as has the role of the audience, but another aspect of the creation of art that has played a significant role in the investigation of what is art is the role of the ideologies and customs inherent in the society in which the artwork is created as well as that which is receiving it, which do not always have similar outlooks. A prime example of this can be found in Michelangelo’s Sistine Chapel. As it was being painted, depiction s of nudes were completely acceptable and natural but only a few years later they were considered indecent and were given flowing garments to cover the more objectionable parts.2 While it has not been agreed upon which is the most important function of art, most all theorists agree that art does convey meaning either cognitively or expressively. The expressive theory of art contends that art conveys its meaning primarily through an appeal to the senses of its audience, evoking specific emotions and feelings intended either consciously or unconsciously by the artist and capable of conveying even complex ideas such as the existence of God or the power of the mind. As defined by Freeland,3 the ‘expression theory’ as it pertains to art is based on the concept that art is intended to communicate something to the viewer that is focused upon exacting an emotion or feeling. This is accomplished through careful use of line

Role Of Icts For Improving Efficiency And Relevance Of Airlines Essay

Role Of Icts For Improving Efficiency And Relevance Of Airlines Services For Tourists - Essay Example In this direction it has become quite crucial for the organisation to undertake some crucial, new and innovative strategic and tactic moves. In this direction adoption of new and competent Information Communication Technologies (ICTs) has become an important and effective way for the business organisations to enhance their overall capabilities in existing challenging business environment (Konig and Strauss 2000). The importance of ICTs can be sighted in different industries across the world. In this context, international airline industry has also been adopted ICTs quite significantly. The proliferation of Internet as an important channel of communication between business organisation and customers has provided some important and effective measures related with ICTs to international Airlines for enhancing quality of services provided to passengers (Sarmento 2011). In direction to this, the paper aims to ICTs relaed trends prevailing within the International Airlines Industry. The paper also elaborates different new technologies and trends related with information and communication technologies (ICT) that are quite prominently followed within airlines industry. The impact and role ICT related practices and trends in advancing customer expectations is also an important point of discussion made in the paper. On the basis of entire findings, the paper also presents some crucial recommendations for airlines industry for using ICT in the more tactical and strategic way so that efficiency and productivity of the industry can be enhanced. ICTs acceptance within airlines industry Like other business industries, the growth of internet and technological advancement of business environment has also affected trends and practices followed in airlines industry. In order to enhance efficiency of services provided to passengers and providing some intensive an d value added experience to them, airlines companies has adopted ICT as one of the most effective tools. The history of ICT acceptance by different Airlines is quite old. Since 1970s, Airlines companies have been using different variants of ICT for facilitating its customers. The availability of telephone availability within this decade has changed the trend of enquiring about availability, reservation and confirmation (Sarmento 2011). In regard to this, first of all American Airlines introduced SABRE Computer Reservation System (CRS) in the year 1962, for the purpose of providing fast and effective facilities regarding reservations and bookings. Afterwards, the system was started to be used for inventory control function within the organisation. This system allowed the company to schedule its crew effectively, track spare parts requirements, and have effective decision support systems for management (Knowles and Garland 1994). The revolution regarding ICTs in Airline industry can b e spotted from mid 1990s. The development of internet and intranet facilities within the market place has allowed the airline industry to have some effective strategies on technological innovations in their ICTs infrastructure. In this regard, British Airways has spent ?1.1 billion on its ICT infrastructure for the purpose of making its distribution channels more effective and responsive. Increasing competitive pressure has imposed business organisations to have enhanced the level of interaction with customers so that their trust and loyalty can be won (Noakes and Coulter 2002). In this concern, effective communication and information technologies used by the company has been considered as effective gateways. In addition to this,